Why is 10^4 used so often?

A 1 mm^2 square at 0.1 mm depth holds 0.0001 mL, and 1 / 0.0001 equals 10,000.

Can I enter more than four squares?

Yes. Enter any number of square counts; the calculator averages them.

Enter chamber counts and dilution factor to calculate cell concentration, total cells, and live cell count.

Answer

1.64E6

Cell concentration

Live cell concentration: 1.64E6 cells/mL

Total cells: 1.64E7

Average count: 82 · Squares counted: 4

Cells per microliter: 1,640

Counts per square

Dilution factorSample volume (mL)Viability (%)Square area (mm^2)Chamber depth (mm)

Conversion factor

10,000

Examples

Count summary

cells/mL = average count x dilution factor / square volume (mL)

For a common 1 mm^2 square with 0.1 mm chamber depth, the square volume is 0.0001 mL, so the conversion factor is 10^4.

Symbol	Variable	Description
Average count	Mean chamber count	Average of the counted squares.
Dilution factor	Dilution	Use 2 for a 1:1 trypan blue mix.
Volume	Square volume	Area x depth converted from mm^3 to mL.

1
Enter counts
Type each counted square separated by commas, spaces, or line breaks.
2
Set dilution and chamber values
The defaults match the common 10^4 hemocytometer factor.
3
Read concentration and totals
Use cells/mL for culture concentration and total cells for the entered sample volume.

Use these values for standard Neubauer-style counting unless your chamber or protocol says otherwise.

Mitotic Index Calculator

Calculate overall and field-level mitotic index from mitotic cell counts and total cell counts across one or more microscope fields.

Hardy-Weinberg Calculator

Calculate allele and genotype frequencies for a population in genetic equilibrium.

DNA GC Content Calculator

Calculate the percentage of guanine and cytosine bases in a DNA sequence.